Biomechanics

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»Book Publisher: CRC Press (25 September, 2007)
»ISBN: 0849385342
»Book author: Donald R. Peterson

Book Description:

Drawn from the third edition of the widely acclaimed The Biomedical Engineering Handbook, Biomechanics is a comprehensive presentation of the current principles and applications of biomechanics integrating both systems and subsystems models. Presenting substantial updates and revisions and a new chapter on current methods and strategies for modeling cellular mechanics, the book covers musculoskeletal mechanics including hard- and soft tissue and joint mechanics, biofluid mechanics and circulatory dynamics, the functioning of the ear, and the performance characteristics of the human body during exercise and exertion. The book includes more than 140 illustrations, 60 tables, and a variety of useful equations to assist in modeling biomechanical behaviors.

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Glycovirology Protocols (Methods in Molecular Biology (Cloth))

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»Book Publisher: Humana Press (2007)
»ISBN: 1588295907
»Book author: Richard J., Ed. Sugrue
»Amazon Rating:

Book Description:

This volume reviews the increasing importance of glycosylation to the field of virology, as well as virus replication. The chapters provide an overview of glycosylation in relation to virus infection, and the generic techniques that are used to analyze and characterize glycoproteins.

Among the topics discussed in this volume are M.E. interaction between respiratory syncytial virus and glycosaminoglycans, acute respiratory syndrome coronavirus 3a protein, the C type lectins DC-SIGN and L-SIGN, N-linked glycans, and the use of two-dimensional SDS-PAGE to analyze the glycosylation heterogeneity of the respiratory syncytial virus fusion protein. Also covered in this volume are uses of monoclonal antibodies and lectins, the spike glycoprotein, secretion of respiratory syncytial vius F protein from insect cells, the expression and characterization of recombinant dengue virus E protein, and cloning, expression, and a functional analysis of patient-derived hepatitis C virus glycoproteins.

The information presented in Glycovirology Protocols will provide insight as to how the techniques of glycobiology can be applied in virology and answer most questions that are of interest to the reader.

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Quantum Dots: Applications in Biology (Methods in Molecular Biology (Cloth))

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»Book Publisher: Humana Press (2007)
»ISBN: 1588295621
»Book author: Marcel P., Ed. Bruchez

Book Description:
Quantum Dots captures many diverse applications enabling utility in biological detection. Organized into five parts, the first two parts cover the use of QDs in imaging fixed and living cells (and tissues). Protocols are included for using QDs in routine (protein and structural cellular labeling), as well as enabling (single receptor trafficking, clinical pathology, correlative microscopy) applications. Part 3 shows early efforts aimed at using QDs in live animals. The final 2 parts demonstrate the versatility of QD technology in existing assay technology.

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In Vitro Transcription and Translation Protocols (Methods in Molecular Biology (Cloth))

Product Details

»Book Publisher: Humana Press (2007)
»ISBN: 1588295583
»Book author: Guido, Ed. Grandi

Book Description:

A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems.In Section One: Technologies, authors discuss the use of alternative energy systems for ATP regeneration, the efficient in vitro production of integral membrane proteins, and the high-throughput production of protein libraries using the innovative single-molecule PRC-linked in vitro expression (SIMPLEX) technology. In Section Two: Applications, authors present in vitro translation of amplified protease gene from HIV patients to select proper anti-protease drug therapy, in vitro translation of genes from human patients to diagnose biologically relevant gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries of randomly produced lipase mutants, and the in vitro transcription and translation to identify proteins in 2D-maps.

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Mass Spectometry Data Analysis in Proteomics (Methods in Molecular Biology Volume 367)

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»Book Publisher: Humana Press Inc. (2007)
»ISBN: 158829563X
»Book author: Rune, Ed. Matthiesen

Book Description:
Mass Spectrometry Data Analysis in Proteomics is an in-depth guide to the theory and practice of analyzing raw mass spectrometry (MS) data in proteomics. As MS is a high throughput technique, proteomic researchers must attend carefully to the associated field of data analysis, and this volume outlines available bioinformatics programs, algorithms, and databases available for MS data analysis. General guidelines for data analysis using search engines such as Mascot, Xtandem, and VEMS are provided, with specific attention to identifying poor quality data and optimizing search parameters. Several different types of MS data are discussed, followed by a description of optimal methods for conversion of raw data into peak lists for input to search engines. Choosing the most accurate and complete databases is emphasized, and a report of available sequence databases is included. Methods for assembling expressed sequence tags (ESTs) into assembled nonredundant databases are provided, along with protocols for further processing the sequences into a format suitable for MS data. Mass Spectrometry Data Analysis in Proteomics describes publicly available applications whenever possible.

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Protocols for Nucleic Acid Analysis by Nonradioactive Probes (Methods in Molecular Biology (Cloth))

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»Book Publisher: Humana Press (2007)
»ISBN: 1588294307
»Book author: Elena, Ed. Hilario

Book Description:
Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results.
The protocols follow the successful Methods in Molecular Biologyâ„¢ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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Protein Engineering Protocols (Methods in Molecular Biology Volume 352)

Product Details
»Book Publisher: Humana Press (2007)
»ISBN: 1588290727
»Book author: Katja M., Ed. Arndt

Book Description:
Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Divided into two sections, Protein Engineering Protocols reviews rational protein design strategies and directed evolutionary techniques, and their impact on protein engineering.
The first section presents design and computational strategies for protein engineering focusing on designing a protein with desired properties, including examples covering a wide range of engineering techniques, such as protein-protein interactions, DNA binding, antibody mimics, and enzymatic activity. The second section on evolutionary techniques provides step-by-step instructions on library design and statistical assessment of library quality. New methods for DNA shuffling as well as different selection strategies are also presented.
Following the successful Methods in Molecular Biologyâ„¢ series format, this volume provides a comprehensive guide to the methods used at every stage of the engineering process. By combining a thorough theoretical foundation with detailed protocols, Protein Engineering Protocols will be invaluable to all research workers in the area, from graduate students to senior investigators.

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Plant Proteomics: Methods and Protocols (Methods in Molecular Biology Volume 355)

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»Book Publisher: Humana Press (2007)
»ISBN: 1588296350
»Book author: Herve, Ed. Thiellement

Book Description:
Plant Proteomics: Methods and Protocols provides up-to-date methods and protocols for plant proteomics, each written by expert researchers from leading laboratories. Experimental methodologies are explained in clear and complete detail for both the wet and dry laboratories. The methods and protocols presented in this volume will inspire the novice as well as the expert researcher to undertake new experimental endeavors in plant proteomics.

Chapters 1 and 2 describe standard protocols and chapters 3 through 10 contain optimized protocols for protein extraction of tissues such as cereal seeds, xylem, phloem sap, of woody plants, and of cellular compartments, such as chloroplasts and mitochondria. These methods rely on improvements to solubilization of proteins, which is the emphasis of Chapters 11 and 12. Chapters 13 to 23 outline methods for protein identification, including 2DLC or LC of intact proteins, mass spectrometry (MALDI-TOF and MS/MS) with proteins separated by 2D gels, blue native ID gels, and Edman sequencing. Chapters 24 to 29 focus on the rapidly expanding area of analysis of post-translational modifications and protein-protein relationships. Attention is also given to data that proteomics produces, including management of that data, and to statistical tools used for effective analysis.

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Metabolomics: Methods and Protocols (Methods in Molecular Biology Volume 358)

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»Book Publisher: Humana Press (2007)
»ISBN: 1588295613
»Book author: Wolfram Weckwerth

Book Description:
Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies.

Part I of this volume focuses on GC-MS techniques; specifically, attention is given to metabolite profiling in blood plasma, construction and application of mass spectral and retention time index libraries, and metabolomic profiling of natural volatiles. In Part II, emphasis shifts to data integration and data mining techniques, and specific protocols discuss improving pattern recognition and biomarker selection for systems level approaches, and visualization and analysis of molecular and metabolomic data. Parts III, IV, and V examine the coupling of capillary electrophoresis and mass spectrometry, classical reversed phase liquid chromatography-mass spectrometry, and electrochemical detection of metabolites using HPLC separations coupled with coulometric electrode array detectors. In Part VI, protocols for the determination of metabolic flux rates are presented and a theoretical framework for metabolic pathway network analysis is provided. The volume concludes with an examination of cutting-edge NMR protocols.

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Cardiovascular Proteomics: Methods and Protocols (Methods in Molecular Biology Volume 357)

Product Details

»Book Publisher: Humana Press (2007)
»ISBN: 1588295354
»Book author: Fernando, Ed. Vivanco

Book Description:
Cardiovascular Proteomics: Methods and Protocols presents cutting-edge protocols and strategies for proteomic evaluation of cardiovascular disease written by pioneering researchers in the field. Topics explored in this comprehensive volume include obtaining specific heart proteins, cutting-edge techniques for identifying risk biomarkers of atherome plaque rupture, analyzing the secretome of explanted endarterectomies cultured in vitro, and phage display techniques for deciphering the molecular diversity of blood vessels. Detailed protocols for the isolation of short- and long-term culture of the adult mouse cardiac myocytes are also included.

Numerous advanced proteomic techniques are addressed, including protein separation by two-dimensional electrophoresis and two-dimensional difference gel electrophoresis, liquid chromatography coupled to mass spectrometry, and the SELDI-TOF approach to searching for biomarkers of stroke in human serum or of hypertension in the serum of animal models. Proteomic strategies and protocols for studying the proteome of endothelial, arterial smooth muscle cells, foam cells, and circulating blood monocytes constitute a major element of this text. Cutting-edge techniques for the analysis of subproteomes from isolation to final characterization and the characterization of posttranslational modifications are also addressed. This useful snapshot of current proteomic techniques is a single source for protocols for the identification and validation of novel biomarkers and targets in cardiovascular diseases.

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Target Discovery and Validation, Vol 2: Reviews and Protocols: Emerging Molecular Targets and Treatment Options ( Methods in Molecular Biology Vol.3 )

Product Details

»Book Publisher: Humana Press (2007)
»ISBN: 1588298906
»Book author: Mouldy, Ed. Sioud

Book Description:
Target Discovery and Validation Reviews and Protocols, Volumes 1 and 2 review the most progressive and current methods for drug target discovery and validation. These volumes explore how recent improvement in understanding the molecular mechanisms of human pathology is impacting drug target discovery in the laboratory and in real therapeutics, specifically for cancers and autoimmune disorders.

Volume 1 focuses on novel and innovative techniques, and presents the most up-to-date protocols available for maximizing the likelihood of achieving target-selective inhibition in vivo while minimizing side effects. The profound impact of genomics, proteomics and bioinformatics on target discovery is explored, and specific attention is given to the role of transgenic and knockout animals in functional genomics and target validation. Cancer researchers will find tremendous value in the molecular classification of breast cancers and the review of protocols for tumor antigens and cancer vaccines. The methods and protocols collected here, all reviewed by leading scientists and clinicians, present the practical details necessary for translating the enormous discovery potential of the genome into real therapeutic products.

Volume 2 collects all the practical details required for efficient translation of discovered targets into real pharmaceutical drugs. Specific targets in cancers and autoimmunity are described and the potential of using siRNAs, antisense oligonucleotides and RNA aptamers in patients is reviewed. This volume explores the tremendous impact of the application of genotyping and gene expression profiling on the future of healthcare, and presents cutting-edge protocols to aid in bringing agents against specific targets closer to application in the clinic.

Collectively, these volumes provide a thorough review of the most cutting-edge methods available for each step in drug target identification, validation, and clinical application. For researchers, an understanding of available methods aids in the creation of innovative experiments in the laboratory, and the successful translation of target discovery to real therapeutics.

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Immunological Tolerance: Methods and Protocols (Methods in Molecular BiologyVolume 380)

Product Details

»Book Publisher: Humana Press (08 June, 2007)
»ISBN: 1588296520
»Book author: Paul J. Fairchild

Book Description:

Immunological Tolerance: Methods and Protocols is a comprehensive guide to the techniques currently used for culturing and characterising the cell types responsible for imposing self-tolerance and the experimental models employed to study their function both in vitro and in vivo.

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YAC Protocols (Methods in Molecular Biology Volume 347)

Product Details
»Book Publisher: Humana Press (2006)
»ISBN: 1588296121
»Book author: Alasdair, Ed. MacKenzie

Book Description:
This completely revised and updated edition of YAC Protocols takes advantage of the many new advances in biology since the publication of the first edition, including the sequencing of the human genome, the genomes of a wide variety of other organisms, and the increased use of transgenic animals for understanding the molecular basis of human and animal disease. This volume also provides a much-needed update on the new techniques currently employed and to help redefine and illustrate the important roles still to be played by yeast artificial chromosome (YAC) technologies in the postgenomic age.
YAC Protocols, Second Edition, demonstrates the enormous potential use of YAC technologies in understanding the role of genetics in maintaining health, promoting pathogenesis, and conferring susceptibility to disease. Offering solutions to the many problems that have plagued researchers, this new edition allows for the wider and more efficient use of YAC technologies for the analysis of genetic and pathogenic causes of human disease.

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Gene Mapping, Discovery, And Expression: Methods And Protocols (Methods in Molecular Biology) (Methods in Molecular Biology Volume 338)

Product Details
»Book Publisher: Humana Press (21 April, 2006)
»ISBN: 1588295753
»Book author: Minou, Ed. Bina

Book Description:
A collection of cutting-edge computational tools and experimental techniques to study how genes are regulated, and to reconstruct the regulatory networks through which various cell-types are produced. On the computational side, web-based technologies to localize genes, to access and retrieve data from microarray databases, to conduct comparative genomics, and to discover the potential “codes” in genomic DNA that may control the expression of protein-coding genes. Detailed experimental techniques described include methods for studying chromatin structure and allele-specific gene expression, methods for high-throughput analysis to characterize the transcription factor binding elements, and methods for isolating and identifying proteins that interact with DNA. The protocols follow the successful Methods in Molecular Biologyâ„¢ series format, each offering step-by-step instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls.

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Checkpoint Controls and Cancer: Volume 2: Activation and Regulation Protocols (Methods in Molecular Biology)

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»Book Publisher: Humana Press (24 June, 2004)
»ISBN: 1588295001
»Book author: Axel H. Schönthal

Book Description:
Prestigious researchers from ten different countries present readily reproducible experimental protocols to study the molecular components of checkpoint controls and their regulation. Described in step-by-step detail, these cutting-edge techniques offer novel approaches, such as the use of genome databases and siRNA, to analyzing how cells of the human body can escape proper surveillance to grow into a tumor. Additional experimental methods are provided for the manipulation of checkpoint pathways and the analysis of the resulting consequences for the cellular phenotype. An earlier first volume, Reviews and Model Systems, comprehensively reviews the complexities of checkpoint controls and the model systems needed to study them.

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The Microbial Models of Molecular Biology: From Genes to Genomes

Product Details
»Book Publisher: Oxford University Press (2003)
»ISBN: 0195154363
»Book author: Rowland H. Davis

Book Description:
This book explains the role of simple biological model systems in the growth of molecular biology. Essentially the whole history of molecular biology is presented here, tracing the work in bacteriophages in E. coli, the role of other prokaryotic systems, and also the protozoan and algal models - Paramecium and Chlamydomonas, primarily - and the move into eukaryotes with the fungal systems - Neurospora, Aspergillus and yeast. Each model was selected for its appropriateness for asking a given class of questions, and each spawned its own community of investigators. Some individuals made the transition to a new model over time, and remnant communities of investigators continue to pursue questions in all these models, as the cutting edge of molecular biological research flowed onward from model to model, and onward into higher organisms and, ultimately, mouse and man.

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Tumor Suppressor Genes: Volume 2: Regulation, Function, and Medicinal Applications (Methods in Molecular Biology)

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»Book Publisher: Humana Press (21 April, 2003)
»ISBN: 0896039870
»Book author: Wafik S. El-Deiry

Book Description:
The second volume of Tumor Suppressor Genes explores the cell biology and biochemical function of the tumor suppressor genes, as well as its physiological role in vivo. The authors detail the physical methods (NMR, microarray approaches, pot-translational structure analysis, analysis of regulation at the gene expression and protein signaling levels)used to understand the function of tumor suppressor genes. In vivo approaches discussed include studies in yeast, Drosophilia, mice, and human tumors.

For both volumes:

Leading physician scientists and academic researchers review all the known tumor suppressor genes, explain how they work, and describe how they were discovered and isolated. In many cases, the authors discuss specific genes that are frequently involved in hereditary or sporadic cancers. They also provide a detailed guide to using powerful molecular genetic, cytogenetic, proteomic, and cell biological strategies to discover and isolate novel tumor suppressor genes and their targets. A second volume of this two-volume set, Tumor Suppressor Genes, Volume 2: Regulation, Function, and Medical Applications, shows how to explore the cell biology and biochemical function of such encoded proteins, to study its physiological role in vivo, and to use information on TSGs to develop diagnostic and therapeutic strategies for cancer.

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Caspases: Their Role in Cell Death and Cell Survival (Molecular Biology Intelligence Unit)

Product Details
»Book Publisher: Springer (31 January, 2003)
»ISBN: 0306474417
»Book author: Marek Los, Henning Walczak

Book Description:
The volume offers insight into the key research topics that involve caspases. Leading experts from various areas of biology and medicine have compiled this volume so that both the novice and the professional will use this as the main reference for caspases for years to come. The reader will learn about the role of caspases in apoptosis signaling, inflammation and cancer therapy while being updated on the methodology used to study caspases and the efforts of pharmaceutical research in targeting caspases.

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Cancer Cell Signaling: Methods and Protocols (Methods in Molecular Biology)

Product Details
»Book Publisher: Humana Press (09 December, 2002)
»ISBN: 1588290751
»Book author: David M. Terrian
»Amazon Rating:

Book Description:
A collection of readily reproducible experimental methods for studying the subversion of normal cell signaling that leads to cancer. Presented in step-by-step detail to ensure successful results, the methods include proven techniques for the investigation of apoptosis and cell death, complementary protocols for manipulating and/or monitoring oncogenic signals in cancer cells, and techniques for studying protein-protein interactions. A detailed protocol for capturing pure samples of malignant cells from frozen tissue specimens and two alternative techniques for analyzing their genomic DNA is also described.

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Oncogene-Directed Therapies (Cancer Drug Discovery and Development)

Product Details
»Book Publisher: Humana Press (03 December, 2002)
»ISBN: 089603982X
»Book author: Janusz W. Rak

Book Description:
Prominent investigators and clinicians summarize in a balanced blend of fundamental science, basic research, experimental therapeutics, and early clinical experiences, what is known about oncogenes and oncogenesis, and describe how that knowledge can be used to treat the cancer. The contributors explain how, why, and under what conditions certain proteins acquire the ability to transform eukaryotic cells, and detail the crucial biological consequences of this oncogenic transformation, particularly for cellular mitogenesis, survival, differentiation, migration, proteolysis, or angiogenic competence. Their articles thoroughly explicate the premises, principles, techniques, and approaches to oncogene targeting in various types of human cancer by using signal transduction inhibitors, immunological targeting methods, and antisense gene therapy.

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