Glycovirology Protocols (Methods in Molecular Biology (Cloth))

Product Details

»Book Publisher: Humana Press (2007)
»ISBN: 1588295907
»Book author: Richard J., Ed. Sugrue
»Amazon Rating:

Book Description:

This volume reviews the increasing importance of glycosylation to the field of virology, as well as virus replication. The chapters provide an overview of glycosylation in relation to virus infection, and the generic techniques that are used to analyze and characterize glycoproteins.

Among the topics discussed in this volume are M.E. interaction between respiratory syncytial virus and glycosaminoglycans, acute respiratory syndrome coronavirus 3a protein, the C type lectins DC-SIGN and L-SIGN, N-linked glycans, and the use of two-dimensional SDS-PAGE to analyze the glycosylation heterogeneity of the respiratory syncytial virus fusion protein. Also covered in this volume are uses of monoclonal antibodies and lectins, the spike glycoprotein, secretion of respiratory syncytial vius F protein from insect cells, the expression and characterization of recombinant dengue virus E protein, and cloning, expression, and a functional analysis of patient-derived hepatitis C virus glycoproteins.

The information presented in Glycovirology Protocols will provide insight as to how the techniques of glycobiology can be applied in virology and answer most questions that are of interest to the reader.

Download Here >>>

Quantum Dots: Applications in Biology (Methods in Molecular Biology (Cloth))

Product Details
»Book Publisher: Humana Press (2007)
»ISBN: 1588295621
»Book author: Marcel P., Ed. Bruchez

Book Description:
Quantum Dots captures many diverse applications enabling utility in biological detection. Organized into five parts, the first two parts cover the use of QDs in imaging fixed and living cells (and tissues). Protocols are included for using QDs in routine (protein and structural cellular labeling), as well as enabling (single receptor trafficking, clinical pathology, correlative microscopy) applications. Part 3 shows early efforts aimed at using QDs in live animals. The final 2 parts demonstrate the versatility of QD technology in existing assay technology.

Download Here >>>

In Vitro Transcription and Translation Protocols (Methods in Molecular Biology (Cloth))

Product Details

»Book Publisher: Humana Press (2007)
»ISBN: 1588295583
»Book author: Guido, Ed. Grandi

Book Description:

A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems.In Section One: Technologies, authors discuss the use of alternative energy systems for ATP regeneration, the efficient in vitro production of integral membrane proteins, and the high-throughput production of protein libraries using the innovative single-molecule PRC-linked in vitro expression (SIMPLEX) technology. In Section Two: Applications, authors present in vitro translation of amplified protease gene from HIV patients to select proper anti-protease drug therapy, in vitro translation of genes from human patients to diagnose biologically relevant gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries of randomly produced lipase mutants, and the in vitro transcription and translation to identify proteins in 2D-maps.

Download Here >>>

Immunological Tolerance: Methods and Protocols (Methods in Molecular BiologyVolume 380)

Product Details

»Book Publisher: Humana Press (08 June, 2007)
»ISBN: 1588296520
»Book author: Paul J. Fairchild

Book Description:

Immunological Tolerance: Methods and Protocols is a comprehensive guide to the techniques currently used for culturing and characterising the cell types responsible for imposing self-tolerance and the experimental models employed to study their function both in vitro and in vivo.

Download Here >>>

Pediatric Hematology: Methods and Protocols (Methods in Molecular Medicine volume 91)

Product Details
»Book Publisher: Humana Press (29 September, 2003)
»ISBN: 1588290433
»Book author: Nicholas J. Goulden, Colin G. Steward

Book Description:
A collection of cutting-edge methods for investigating and detecting a wide variety of hematological disorders. Here, the reader will find reliable molecular protocols for the diagnosis of Fanconi anemia and dyskeratosis congenita, immunodeficiency, and most forms of hemoglobinopathy. In addition, there are detailed methods for molecular human platelet antigen genotyping, an effective PCR procedure for thrombophilia screening, and protocols for fluorescent in situ hybridization. Since the measurement of minimal residual disease (MRD) provides a much more accurate risk-directed therapy, three methods are presented for detecting residual leukemia below the threshold of light microscopy, along with relatively simple, rapid, and cheap methods for the detection of MRD in ALL and AML.

Download Here >>>