Biomechanics

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»Book Publisher: CRC Press (25 September, 2007)
»ISBN: 0849385342
»Book author: Donald R. Peterson

Book Description:

Drawn from the third edition of the widely acclaimed The Biomedical Engineering Handbook, Biomechanics is a comprehensive presentation of the current principles and applications of biomechanics integrating both systems and subsystems models. Presenting substantial updates and revisions and a new chapter on current methods and strategies for modeling cellular mechanics, the book covers musculoskeletal mechanics including hard- and soft tissue and joint mechanics, biofluid mechanics and circulatory dynamics, the functioning of the ear, and the performance characteristics of the human body during exercise and exertion. The book includes more than 140 illustrations, 60 tables, and a variety of useful equations to assist in modeling biomechanical behaviors.

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Glycovirology Protocols (Methods in Molecular Biology (Cloth))

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»Book Publisher: Humana Press (2007)
»ISBN: 1588295907
»Book author: Richard J., Ed. Sugrue
»Amazon Rating:

Book Description:

This volume reviews the increasing importance of glycosylation to the field of virology, as well as virus replication. The chapters provide an overview of glycosylation in relation to virus infection, and the generic techniques that are used to analyze and characterize glycoproteins.

Among the topics discussed in this volume are M.E. interaction between respiratory syncytial virus and glycosaminoglycans, acute respiratory syndrome coronavirus 3a protein, the C type lectins DC-SIGN and L-SIGN, N-linked glycans, and the use of two-dimensional SDS-PAGE to analyze the glycosylation heterogeneity of the respiratory syncytial virus fusion protein. Also covered in this volume are uses of monoclonal antibodies and lectins, the spike glycoprotein, secretion of respiratory syncytial vius F protein from insect cells, the expression and characterization of recombinant dengue virus E protein, and cloning, expression, and a functional analysis of patient-derived hepatitis C virus glycoproteins.

The information presented in Glycovirology Protocols will provide insight as to how the techniques of glycobiology can be applied in virology and answer most questions that are of interest to the reader.

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Quantum Dots: Applications in Biology (Methods in Molecular Biology (Cloth))

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»Book Publisher: Humana Press (2007)
»ISBN: 1588295621
»Book author: Marcel P., Ed. Bruchez

Book Description:
Quantum Dots captures many diverse applications enabling utility in biological detection. Organized into five parts, the first two parts cover the use of QDs in imaging fixed and living cells (and tissues). Protocols are included for using QDs in routine (protein and structural cellular labeling), as well as enabling (single receptor trafficking, clinical pathology, correlative microscopy) applications. Part 3 shows early efforts aimed at using QDs in live animals. The final 2 parts demonstrate the versatility of QD technology in existing assay technology.

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In Vitro Transcription and Translation Protocols (Methods in Molecular Biology (Cloth))

Product Details

»Book Publisher: Humana Press (2007)
»ISBN: 1588295583
»Book author: Guido, Ed. Grandi

Book Description:

A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems.In Section One: Technologies, authors discuss the use of alternative energy systems for ATP regeneration, the efficient in vitro production of integral membrane proteins, and the high-throughput production of protein libraries using the innovative single-molecule PRC-linked in vitro expression (SIMPLEX) technology. In Section Two: Applications, authors present in vitro translation of amplified protease gene from HIV patients to select proper anti-protease drug therapy, in vitro translation of genes from human patients to diagnose biologically relevant gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries of randomly produced lipase mutants, and the in vitro transcription and translation to identify proteins in 2D-maps.

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Mass Spectometry Data Analysis in Proteomics (Methods in Molecular Biology Volume 367)

Product Details
»Book Publisher: Humana Press Inc. (2007)
»ISBN: 158829563X
»Book author: Rune, Ed. Matthiesen

Book Description:
Mass Spectrometry Data Analysis in Proteomics is an in-depth guide to the theory and practice of analyzing raw mass spectrometry (MS) data in proteomics. As MS is a high throughput technique, proteomic researchers must attend carefully to the associated field of data analysis, and this volume outlines available bioinformatics programs, algorithms, and databases available for MS data analysis. General guidelines for data analysis using search engines such as Mascot, Xtandem, and VEMS are provided, with specific attention to identifying poor quality data and optimizing search parameters. Several different types of MS data are discussed, followed by a description of optimal methods for conversion of raw data into peak lists for input to search engines. Choosing the most accurate and complete databases is emphasized, and a report of available sequence databases is included. Methods for assembling expressed sequence tags (ESTs) into assembled nonredundant databases are provided, along with protocols for further processing the sequences into a format suitable for MS data. Mass Spectrometry Data Analysis in Proteomics describes publicly available applications whenever possible.

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Protocols for Nucleic Acid Analysis by Nonradioactive Probes (Methods in Molecular Biology (Cloth))

Product Details
»Book Publisher: Humana Press (2007)
»ISBN: 1588294307
»Book author: Elena, Ed. Hilario

Book Description:
Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results.
The protocols follow the successful Methods in Molecular Biologyâ„¢ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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Protein Engineering Protocols (Methods in Molecular Biology Volume 352)

Product Details
»Book Publisher: Humana Press (2007)
»ISBN: 1588290727
»Book author: Katja M., Ed. Arndt

Book Description:
Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Divided into two sections, Protein Engineering Protocols reviews rational protein design strategies and directed evolutionary techniques, and their impact on protein engineering.
The first section presents design and computational strategies for protein engineering focusing on designing a protein with desired properties, including examples covering a wide range of engineering techniques, such as protein-protein interactions, DNA binding, antibody mimics, and enzymatic activity. The second section on evolutionary techniques provides step-by-step instructions on library design and statistical assessment of library quality. New methods for DNA shuffling as well as different selection strategies are also presented.
Following the successful Methods in Molecular Biologyâ„¢ series format, this volume provides a comprehensive guide to the methods used at every stage of the engineering process. By combining a thorough theoretical foundation with detailed protocols, Protein Engineering Protocols will be invaluable to all research workers in the area, from graduate students to senior investigators.

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